The Sekelsky Lab

pP{BeloBAC}

pP{BeloBAC} is a derivative of pBeloBAC11, a vector that is maintained at about one copy per cell. The low copy number greatly increases the stability of cloned repetitive DNA by eliminating the opportunity for inter-molecular recombination events. The tradeoff is that DNA yields are very low. This vector should be grown in a strain that does not already harbor a BAC; we use STBL cells (Gibco/Life Technologies). This derivative has P element ends and some unique cloning sites, but no marker for transformation (I thought about putting one in, but BAC vectors are such a pain to deal with, I just didn't get to it).

pP{BeloBAC} Sequence

CATGATGAAATAACATAAGGTGGTCCCGTCGGCAAGAGACATCCACTTAACGTATGCTT GCAATAAGTGCGAGTGAAAGGAATAGTATTCTGAGTGTCGTATTGAGTCTGAGTGAGAC AGCGATATGATTGTTGATTAACCCTTAGCATGTCCGTGGGGTTTGAATTAACTCATAAT ATTAATTAGACGAAATTATTTTTAAAGTTTTATTTTTAATAATTTGCGAGTACGCAAAg ctagtatGGCGGCCGCGGATCCAGTGCAGTAAAAAAAAAAAATGTTTTTTTTATCTACT TTCCGCAAAAATGGGTTTTATTAACTTACATACATACTAGAATTGCAAAACGTCGTGAG ACAGTTTGGTGCCTACGCGTCCGCGGGACTAGTGCTAGCCATGCGTTTAAACctagtaa ttaattcggctgctgctctAAACGACGCATTTCGTACTCCAAAGTACGAATTTTTTCCC TCAAGCTCTTATTTTCATTAAACAATGAACAGGACCTAACGCACAGTCACGTTATTGTT TACATAAATGATTTTTTTTACTATTCAAACTTACTCTGTTTGTGTACTCCCACTGGTAT AGCCTTCTTTTATCTTTTCTGGTTCAGGCTCTATCACTTTACTAGGTACGGCATCTGCG TTGAGTCGCCTCCTTTTAAATGTCTGACCTTTTGCAGGTGCAGCCTTCCACTGCGAATC ATTAAAGTGGGTATCACAAATTTGGGAGTTTTCACCAAGGCTGCACCCAAGGCTCTGCT CCCACAATTTTCTCTTAATAGCACACTTCGGCACGTGAATTAATTTTACTCCAGTCACA GCTTTGCAGCAAAATTTGCAATATTTCATTTTTTTTTATTCCACGTAAGGGTTAATGTT TTCAAAAAAAAATTCGTCCGCACACAACCTTTCCTCTCAACAAGCAAACGTGCACTGAA TTTAAGTGTATACTTCGGTAAGCTTCGGCTTTCGACGGGACCACCTTATGTTATTTCAT CATGggccagacccacgtagtccatcggccgcccgggccgtcgaccaattctcatgttt gacagcttatcatcgaatttctgccattcatccgcttattatcacttattcaggcgtag caaccaggcgtttaagggcaccaataactgccttaaaaaaattacgccccgccctgcca ctcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacaaa cggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatat ttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaa actggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctt tagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtaga aactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctc atggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttca ttgccatacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggcc ggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctg aacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttac gatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagct tccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttc attatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagt tggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtga tcttccgtcacaggtatttattcgcgataagctcatggagcggcgtaaccgtcgcacag gaaggacagagaaagcgcggatctgggaagtgacggacagaacggtcaggacctggatt ggggaggcggttgccgccgctgctgctgacggtgtgacgttctctgttccggtcacacc acatacgttccgccattcctatgcgatgcacatgctgtatgccggtataccgctgaaag ttctgcaaagcctgatgggacataagtccatcagttcaacggaagtctacacgaaggtt tttgcgctggatgtggctgcccggcaccgggtgcagtttgcgatgccggagtctgatgc ggttgcgatgctgaaacaattatcctgagaataaatgccttggcctttatatggaaatg tggaactgagtggatatgctgtttttgtctgttaaacagagaagctggctgttatccac tgagaagcgaacgaaacagtcgggaaaatctcccattatcgtagagatccgcattatta atctcaggagcctgtgtagcgtttataggaagtagtgttctgtcatgatgcctgcaagc ggtaacgaaaacgatttgaatatgccttcaggaacaatagaaatcttcgtgcggtgtta cgttgaagtggagcggattatgtcagcaatggacagaacaacctaatgaacacagaacc atgatgtggtctgtccttttacagccagtagtgctcgccgcagtcgagcgacagggcga agccctcgagtgagcgaggaagcaccagggaacagcacttatatattctgcttacacac gatgcctgaaaaaacttcccttggggttatccacttatccacggggatatttttataat tattttttttatagtttttagatcttcttttttagagcgccttgtaggcctttatccat gctggttctagagaaggtgttgtgacaaattgccctttcagtgtgacaaatcaccctca aatgacagtcctgtctgtgacaaattgcccttaaccctgtgacaaattgccctcagaag aagctgttttttcacaaagttatccctgcttattgactcttttttatttagtgtgacaa tctaaaaacttgtcacacttcacatggatctgtcatggcggaaacagcggttatcaatc acaagaaacgtaaaaatagcccgcgaatcgtccagtcaaacgacctcactgaggcggca tatagtctctcccgggatcaaaaacgtatgctgtatctgttcgttgaccagatcagaaa atctgatggcaccctacaggaacatgacggtatctgcgagatccatgttgctaaatatg ctgaaatattcggattgacctctgcggaagccagtaaggatatacggcaggcattgaag agtttcgcggggaaggaagtggttttttatcgccctgaagaggatgccggcgatgaaaa aggctatgaatcttttccttggtttatcaaacgtgcgcacagtccatccagagggcttt acagtgtacatatcaacccatatctcattcccttctttatcgggttacagaaccggttt acgcagtttcggcttagtgaaacaaaagaaatcaccaatccgtatgccatgcgtttata cgaatccctgtgtcagtatcgtaagccggatggctcaggcatcgtctctctgaaaatcg actggatcatagagcgttaccagctgcctcaaagttaccagcgtatgcctgacttccgc cgccgcttcctgcaggtctgtgttaatgagatcaacagcagaactccaatgcgcctctc atacattgagaaaaagaaaggccgccagacgactcatatcgtattttccttccgcgata tcacttccatgacgacaggatagtctgagggttatctgtcacagatttgagggtggttc gtcacatttgttctgacctactgagggtaatttgtcacagttttgctgtttccttcagc ctgcatggattttctcatactttttgaactgtaatttttaaggaagccaaatttgaggg cagtttgtcacagttgatttccttctctttcccttcgtcatgtgacctgatatcggggg ttagttcgtcatcattgatgagggttgattatcacagtttattactctgaattggctat ccgcgtgtgtacctctacctggagtttttcccacggtggatatttcttcttgcgctgag cgtaagagctatctgacagaacagttcttctttgcttcctcgccagttcgctcgctatg ctcggttacacggctgcggcgagcgctagtgataataagtgactgaggtatgtgctctt cttatctccttttgtagtgttgctcttattttaaacaactttgcggttttttgatgact ttgcgattttgttgttgctttgcagtaaattgcaagatttaataaaaaaacgcaaagca atgattaaaggatgttcagaatgaaactcatggaaacacttaaccagtgcataaacgct ggtcatgaaatgacgaaggctatcgccattgcacagtttaatgatgacagcccggaagc gaggaaaataacccggcgctggagaataggtgaagcagcggatttagttggggtttctt ctcaggctatcagagatgccgagaaagcagggcgactaccgcacccggatatggaaatt cgaggacgggttgagcaacgtgttggttatacaattgaacaaattaatcatatgcgtga tgtgtttggtacgcgattgcgacgtgctgaagacgtatttccaccggtgatcggggttg ctgcccataaaggtggcgtttacaaaacctcagtttctgttcatcttgctcaggatctg gctctgaaggggctacgtgttttgctcgtggaaggtaacgacccccagggaacagcctc aatgtatcacggatgggtaccagatcttcatattcatgcagaagacactctcctgcctt tctatcttggggaaaaggacgatgtcacttatgcaataaagcccacttgctggccgggg cttgacattattccttcctgtctggctctgcaccgtattgaaactgagttaatgggcaa atttgatgaaggtaaactgcccaccgatccacacctgatgctccgactggccattgaaa ctgttgctcatgactatgatgtcatagttattgacagcgcgcctaacctgggtatcggc acgattaatgtcgtatgtgctgctgatgtgctgattgttcccacgcctgctgagttgtt tgactacacctccgcactgcagtttttcgatatgcttcgtgatctgctcaagaacgttg atcttaaagggttcgagcctgatgtacgtattttgcttaccaaatacagcaatagtaat ggctctcagtccccgtggatggaggagcaaattcgggatgcctggggaagcatggttct aaaaaatgttgtacgtgaaacggatgaagttggtaaaggtcagatccggatgagaactg tttttgaacaggccattgatcaacgctcttcaactggtgcctggagaaatgctctttct atttgggaacctgtctgcaatgaaattttcgatcgtctgattaaaccacgctgggagat tagataatgaagcgtgcgcctgttattccaaaacatacgctcaatactcaaccggttga agatacttcgttatcgacaccagctgccccgatggtggattcgttaattgcgcgcgtag gagtaatggctcgcggtaatgccattactttgcctgtatgtggtcgggatgtgaagttt actcttgaagtgctccggggtgatagtgttgagaagacctctcgggtatggtcaggtaa tgaacgtgaccaggagctgcttactgaggacgcactggatgatctcatcccttcttttc tactgactggtcaacagacaccggcgttcggtcgaagagtatctggtgtcatagaaatt gccgatgggagtcgccgtcgtaaagctgctgcacttaccgaaagtgattatcgtgttct ggttggcgagctggatgatgagcagatggctgcattatccagattgggtaacgattatc gcccaacaagtgcttatgaacgtggtcagcgttatgcaagccgattgcagaatgaattt gctggaaatatttctgcgctggctgatgcggaaaatatttcacgtaagattattacccg ctgtatcaacaccgccaaattgcctaaatcagttgttgctcttttttctcaccccggtg aactatctgcccggtcaggtgatgcacttcaaaaagcctttacagataaagaggaatta cttaagcagcaggcatctaaccttcatgagcagaaaaaagctggggtgatatttgaagc tgaagaagttatcactcttttaacttctgtgcttaaaacgtcatctgcatcaagaacta gtttaagctcacgacatcagtttgctcctggagcgacagtattgtataagggcgataaa atggtgcttaacctggacaggtctcgtgttccaactgagtgtatagagaaaattgaggc cattcttaaggaacttgaaaagccagcaccctgatgcgaccacgttttagtctacgttt atctgtctttacttaatgtcctttgttacaggccagaaagcataactggcctgaatatt ctctctgggcccactgttccacttgtatcgtcggtctgataatcagactgggaccacgg tcccactcgtatcgtcggtctgattattagtctgggaccacggtcccactcgtatcgtc ggtctgattattagtctgggaccacggtcccactcgtatcgtcggtctgataatcagac tgggaccacggtcccactcgtatcgtcggtctgattattagtctgggaccatggtccca ctcgtatcgtcggtctgattattagtctgggaccacggtcccactcgtatcgtcggtct gattattagtctggaaccacggtcccactcgtatcgtcggtctgattattagtctggga ccacggtcccactcgtatcgtcggtctgattattagtctgggaccacgatcccactcgt gttgtcggtctgattatcggtctgggaccacggtcccacttgtattgtcgatcagacta tcagcgtgagactacgattccatcaatgcctgtcaagggcaagtattgacatgtcgtcg taacctgtagaacggagtaacctcggtgtgcggttgtatgcctgctgtggattgctgct gtgtcctgcttatccacaacattttgcgcacggttatgtggacaaaatacctggttacc caggccgtgccggcacgttaaccgggctgcatccgatgcaagtgtgtcgctgtcgacga
gctcacgatgagaatggccagac

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